Plasmid_Backbone

Part:BBa_K1444028:Design

Designed by: Dave Curran   Group: iGEM14_Calgary   (2014-10-17)

pET28b+ cloning vector from BioBasic


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 192
    Illegal XbaI site found at 334
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 192
    Illegal NheI site found at 230
    Illegal NotI site found at 165
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 192
    Illegal BglII site found at 400
    Illegal BamHI site found at 198
    Illegal XhoI site found at 158
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 192
    Illegal XbaI site found at 334
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 192
    Illegal XbaI site found at 334
    Illegal NgoMIV site found at 432
    Illegal NgoMIV site found at 2020
    Illegal NgoMIV site found at 2180
    Illegal NgoMIV site found at 5227
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal SapI site found at 3100


Design Notes

This plasmid contains kanamycin resistance, and is a high copy-number vector.


Source

This cloning vector came from the gene synthesis services offered by [http://store.biobasic.com/gene-synthesis/ BioBasic].

References